Theory And Mechanisms Of Staining
Theory and Mechanisms of Staining (9815-08)
Review the theory and mechanisms of staining, dye classification and tissue demonstration methods and improve your skills and the quality of prepared material.
| Code |
9815-08 |
| Niveau |
Supérieur |
Prix membres
$0
Prix non-membres
$135
Renseignements additionnels
PEP hours: 12
CPS/ART credits: 0.5
Learning Outcomes:
- Define the term resonance system in relation to dye chemistry
- Define the following terms in relation to dye structure and classification: chromophore (quinoid ring, azo coupling, nitro grouping), chromogen, auxochrome (hydroxyl, amine, carboxyl, sulphonic)
- Relate acid, basic and neutral dyes to their staining properties
- Discuss physical theories of staining: selective solubility, adsorption, porosity
- Describe chemical theories of staining: salt linkage, hydrogen bonding, metachromasia, leukostaining (leucostaining), metallic impregnation, histochemical reactions
- Describe proper storage of dyes and indicate criteria
- Define colour index number
- Differentiate between progressive and regressive staining and state advantages and disadvantages of each
- Classify dyes according to origin and examples of each
- Explain the principle and use of direct and indirect staining
- Describe use of accelerators and accentuators. Define the terms.
- State principle and application of argyrophil and argentaffin reactions
- Name three (3) chromophoric groups found in many commonly used stains, show the basic chemical structure and name at least one dye that contains the group
- Define: Basophilic tissue, Acidophilic tissue
- Define: Mordant, Lake, Accentuator, Accelerator, Metachromasia, Leuco compound, Chromotrope, Orthochromasia
Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: September 2008