Neisseria And Moraxella
Neisseria and Moraxella (9821-12)
Learn the morphology, growth requirements, identification tests and susceptibility patterns, and pathogenicity in order to better differentiate Neisseria species including N. gonorrhoeae, N. meningitides and Moraxella catarrhalis.
| Code |
9821-12 |
| Niveau |
Fondamental |
Prix membres
$0
Prix non-membres
$135
Renseignements additionnels
PEP hours: To be assigned
CPS/ART credits: To be assigned
Learning Outcomes:
Neisseria gonorrhoeae
· Describe typical cellular morphology in direct smears and cultures.
· State the significance of finding intracellular bacteria in urethral exudate.
· State the growth requirements
· Explain how auxotyping is performed and why this may be done.
· Name suitable media for isolation. Explain the usual reason why a strain of N. gonorrhoeae may fail to grow on selective media.
· Explain the difference between a holding and growing transport medium.
· Describe culture techniques used to enhance growth of N. gonorrhoeae in culture media.
· State the criteria used to establish the genus Neisseria.
· List the five types of tests used for identification and know the reactions for N. gonorrhoeae
· Name the two types of serological tests available for identification of N. gonorrhoeae.
· Describe the advantage of a gene probe technique in diagnosis of gonorrhea.
· Describe the changing susceptibility pattern for N. gonorrhoeae. Describe the two resistance mechanisms to penicillin and state how each is detected.
· Describe clinical infections caused by N. gonorrhoeae.
Neisseria meningitidis
· Describe typical cellular morphology.
· Describe growth requirements and typical colonies on blood agar.
· State reactions in carbohydrate utilization tests. Name two other test systems that may be used for identification.
· State the usual susceptibility to penicillin.
· State where N. meningitidis is found in carriers.
· Name the two infections commonly caused by N. meningitidis.
· Describe typical findings in spinal fluid from cases of meningococcal meningitidis and state how antigen can be detected directly from spinal fluid.
Neisseria species
· Describe typical cellular morphology.
· State the growth requirements
· Describe colonial morphology typical of Neisseria species.
· State when definitive identification is required and how this is accomplished (not specific reactions).
· For N. lactamica, know what type of media it grows on and the reactions in the carbohydrate utilization tests.
· Explain how N. polysaccharea and N. subflava could be confused with N. meningitidis and how they may be differentiated using biochemical tests.
· Explain why N. cinerea may be wrongly identified as N. gonorrhoeae and how to avoid this problem.
· State where Neisseria species are found as normal flora and types of infections reported.
Moraxella catarrhalis
· State the three names previously used for this organism.
· Describe typical cellular morphology.
· Describe growth requirements. Describe typical colonies on blood agar.
· State reactions in carbohydrate utilization tests and state how differentiation from Neisseria species is accomplished.
· State how and why beta-lactamase testing should be done.
· List common infections caused by M. catarrhalis.
Oxidase Test
· Outline the principle of the test.
· Name the active ingredient in oxidase reagent.
· Explain how the reagent is stabilized and how this affects stability and sensitivity.
· Describe the filter paper method
· State when quality control organisms should be tested and name suitable control organisms.
Instructor: Helen Smith, MLT
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: January 2012