Introduction To Anaerobes
Introduction to Anaerobes (9832-12)
Learn how to more readily identify species of Veillonella, Peptostreptococcus, Propionibacterium, Clostridium, Bacteroides and Fusobacterium.
| Code |
9832-12 |
| Niveau |
Supérieur |
Prix membres
$0
Prix non-membres
$135
Renseignements additionnels
PEP hours: 11
CPS/ART Credits: 0
Learning Outcomes:
· Define the following according to their atmospheric requirements:
· Obligate anaerobe
· Microaerophilic
· Facultative anaerobe
· List the genera of common anaerobes found in each of the following groups:
· Gram positive cocci
· Gram negative cocci
· Gram negative bacilli
· Non-spore forming Gram positive rods
· Spore forming Gram positive rods
· List the six anaerobes that account for over 90% of clinically significant isolates of anaerobes.
· Explain why knowledge of normal flora anaerobes is essential when performing anaerobic microbiology.
· State the origin of the bacteria usually responsible for anaerobic infections.
· List characteristics that indicate a possible anaerobic infection.
· List specimens that should be routinely rejected for anaerobic culture.
· Describe the anaerobic transport tube, anaerobic bag and anaerobic tube and explain how each can be used to transport anaerobic specimens.
· Define PRAS media and state advantage of using these.
· List four media recommended for primary isolation of anaerobes and explain the function of each selective medium.
· List two liquid media that may be used for growing anaerobes.
· Explain how anaerobic conditions are established for incubation of anaerobes.
· Understand how an anaerobic atmosphere is created, maintained and monitored. Name the organism used as a biological check of anaerobic conditions.
· Describe the processing and incubation of anaerobic specimens.
· State how the Gram stain procedure should be modified for anaerobic work.
· Describe the routine work up of a colony suspected of being an anaerobe.
· List the three special potency antibiotic discs that may be added to anaerobic subcultures and explain how to interpret the results.
· Briefly describe the tests used for presumptive identification of anaerobes
· List the two types of tests required for definitive identification of anaerobes.
· State the method of antimicrobial susceptibility testing recommended by the CLSI (formerly NCCLS).
· State how beta-lactamase testing should be done on anaerobes and the interpretation of a positive result.
· Explain the rationale for not performing susceptibility testing on all anaerobes.
· Describe the cellular morphology and presumptive identification of Peptostreptococcus anaerobius and Peptostreptococcus asaccharolyticus.
· Describe the presumptive identification of Propionibacterium acnes and explain the significance when isolated from blood cultures.
· Describe the presumptive identification of Clostridium perfringens and the pathogenicity of this organism.
· Name the organism often responsible for pseudomembranous colitis and state the usual method of diagnosis.
· State the incidence of isolation of Bacteroides fragilis group and why BBE is a useful primary isolation medium.
· Describe presumptive identification of Bacteroides fragilis group and state susceptibility to penicillin.
· List the two genera of anaerobes that may show pigmented colonies on blood agar.
· Describe presumptive identification of Fusobacterium nucleatum.
Instructor: Helen Smith, MLT
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: January 2012