Hematoxylin And Eosin (H&E) Stain
Hematoxylin And Eosin (H&E) Stain (9816-08)
Reinforce your understanding of the H&E stain by examining this stain in detail in terms of theory, practice, category differentiation and trouble-shooting "when things go wrong".
| Code |
9816-08 |
| Niveau |
Supérieur |
Prix membres
$0
Prix non-membres
$135
Renseignements additionnels
PEP hours: 9
CPS/ART credits: 0.4
Learning Outcomes:
· Describe the stain theory principles involved in the H&E stain.
· Name the source of the natural stain "hematoxylin".
· Differentiate between hematoxylin and hematein.
· Name 4 chemicals used as mordants.
· Define "alum".
· Describe the colour produced by the different mordants with hematoxylin.
· Name four (4) chemicals used as oxidants.
· Define oxidation ("ripening") of hematoxylin.
· Define/discuss and distinguish between regressive and progressive hematoxylin staining
· Discuss blueing of hematoxylin stained sections under the following headings:
a. Principle
b.Mechanism
c. Result
d.Reagents used.
e.pH
· Classify the dye group to which eosin belongs
· Differentiate the dye as anionic or cationic
· Name three (3) other commonly used dyes that belong to the same dye category as eosin.
· Differentiate between eosin Y and eosin B.
· Discuss properties, advantages, disadvantages, use and chemical composition of Harris, Mayer, Weigert, Verhoeff and Gill hematoxylin.
· State a cause and suggest corrective technique for the following problems that may occur with hematoxylin staining:
a. Uniformly weak staining of nuclei
b.Nuclei dark blue with poor detail and cytoplasm is grey-blue.
c. Dark granules (purple) have precipitated on the sections
d.Nuclei are stained dull red, or brown
· State a cause and suggest corrective technique for the following problems that may occur during eosin staining:
a. Uniformly weak staining of cytoplasm
b.Nuclei dark blue with poor detail and cytoplasm is grey-blue
c. Dark staining with eosin that obscures the nuclear stain
d.Nuclei are stained dull red, or brown
· Define colour index number and state the purpose for its use.
Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: September 2008