Fixation Practices
Fixation Practices (9893-09)
Learn how to categorize different types of fixing agents; to elaborate on concepts in fixation and preservation of cellular elements for different types of analysis; and to utilize principles of tissue/cell preservation and fixation in order to effectively demonstrate specific components of cells and tissues.
| Code |
9893-09 |
| Niveau |
Fondamental |
Prix membres
$0
Prix non-membres
$135
Renseignements additionnels
PEP hours: 11
CPS/ART credits: 0
Learning Outcomes:
- Describe basic structure and reactions of protein in relation to fixation
- Define purpose/function of fixation
- Identify chemical structure of acetone, formaldehyde, ethanol, formic acid, picric acid, methanol, acetic acid, mercuric chloride, glutaraldehyde, osmium tetroxide, potassium dichromate, glyoxal
- Describe features of fixing agents
- Define preservation and differentiate from fixation.
- Identify fixation/fixative artifacts
- Describe the effects and optimization of temperature, volume of fixative, osmolality, concentration, time of exposure and [H+] concentration on fixation of tissue
- Describe how the size of tissue affects fixation
- State the optimal thickness of tissue for paraffin processing for light microscopy
- State the optimal thickness of tissue for electron microscopy
- Differentiate between non-coagulant and coagulant fixative
- Describe the mode of action and general classification, advantages, disadvantages and safety precautions associated with: formaldehyde, ethanol and alcohol solutions, picric acid, osmium tetroxide, mercuric chloride
- Describe conditions/analyses when/where fresh, unfixed tissue is required
- Define/Describe the use of post-fixation
- Discuss the theories regarding mechanisms of various types of fixatives
Instructor: Masaye Tanaka, MLT, ART, BSc, MEd
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: December 2009