Fastidious Gram Negative Bacilli
Fastidious Gram Negative Bacilli (9820-11)
Learn about the classification and nomenclature, morphology and growth requirements, identification and susceptibility, pathogenicity and relationship to differentiation strategies for Haemophilus, Bordetella, Pasteurella, Gardnerella and Capnocytophaga.
| Code |
9820-11 |
| Niveau |
Supérieur |
Prix membres
$0
Prix non-membres
$135
Renseignements additionnels
PEP hours: 11
CPS/ART credits: 0
Learning Outcomes:
· State the criteria originally used to place an organism in the genus Haemophilus.
Haemophilus influenzae
· Describe the cellular morphology.
· Describe the growth requirements:
· Describe colonial morphology on blood agar with a staph streak and on chocolate agar.
· State the criteria necessary to establish the genus Haemophilus.
· State how to differentiate the different species of Haemophilus.using hemolysis, growth factor requirements and porphyrin test.
· Explain why biotyping and serotyping would be carried out and state which serotype is the most virulent.
· State the approximate rate of resistance to ampicillin, describe the two mechanisms responsible for the resistance and how each is detected.
· Name a rapid test for chloramphenicol resistance.
· Explain why childhood infections due to this organism are decreasing in number.
· Name the two invasive infections found in children and describe typical findings in spinal fluid from a case of meningitis.
Haemophilus aegyptius
· Explain the taxonomic relationship with H. influenzae.
· Describe two acute infections caused by H. aegyptius.
Other Haemophilus species
· Describe the clinical significance of H. parainfluenzae, H. haemolyticus and H. parahaemolyticus.
· State the atmosphere required for isolation of H. aphrophilus and the type of specimen where it may be found as a pathogen.
· Describe the cellular morphology of H. ducreyi in direct smears and in cultures.
· State the pathogenicity of H. ducreyi.
X and V Growth Factor Requirements
· State the criteria the test medium must meet and name the recommended medium.
· State how the test medium should be inoculated, how carryover of growth factors in the inoculum would affect results and how to avoid this.
· Describe how to interpret results.
Porphyrin Test
· State the principle of the test including the substrate used.
· State how the test is inoculated and describe two methods of detecting porphyrins.
· State how to interpret test results.
· Explain how the test interpretation is affected by:
· Testing bacteria belonging to another genus
· Using non-viable bacteria
· Using too light an inoculum
Bordetella
· Name the species most commonly infecting man.
Bordetella pertussis
· Describe typical cellular morphology.
· Describe how diagnosis may be made using direct smears.
· Name the site that should be sampled for culture and explain how the specimen is obtained.
· Name and describe a suitable transport medium.
· Describe the growth requirements. Name two media used for isolation of B. pertussis, compare the shelf life and name the antibiotic recommended for these media.
· State the usual time required for visible colonies to form.
· State the criteria indicating the genus Bordetella.
· State how to differentiate B. pertussis from B. parapertussis and B. bronchiseptica.
· Name the disease caused by B. pertussis and describe the symptoms.
· Describe the clinical significance of B. parapertussis and B. bronchiseptica.
Capnocytophaga
· Describe typical cellular morphology.
· State suitable media and atmosphere for growth.
· Describe typical colonies.
· Describe laboratory findings that indicate Capnocytophaga.
· State how complete species identification is carried out.
· Describe the pathogenicity of C. canimorsus and state the usual source of the bacteria.
Actinobacillus
· Describe typical cellular morphology.
· State suitable media and atmosphere for growth.
· Describe typical colonies.
· Describe laboratory findings that indicate Actinobacillus
· Describe the pathogenicity of Actinobacillus and state the usual source of the bacteria.
Eikenella
· Describe typical cellular morphology.
· State suitable media and atmosphere for growth.
· Describe typical colonies.
· Describe laboratory findings that indicate Eikenella
· Describe the pathogenicity of Eikenella and state the usual source of the bacteria.
Kingella
· Describe typical cellular morphology.
· State suitable media and atmosphere for growth.
· Describe typical colonies.
· Describe laboratory findings that indicate Kingella.
· Describe the pathogenicity of Kingella and state the usual source of the bacteria.
Cardibacterium
- Describe typical cellular and colonial morphology.
- Describe pathogenicity
Pasteurella
· Name the species most commonly isolated from man.
· Name the genus to which some Pasteurella species are being moved.
Pasteurella multocida
· Describe typical cellular morphology.
· Describe growth requirements:
· Describe typical colonies on blood agar.
· State criteria that indicate a possible P. multocida.State the usual source of the bacteria infecting man and describe clinical infection.
Instructor: Helen Smith, MLT
Prerequisites: None
Textbook: N/A
Equipment: Computer with Internet is required
Start Date: Upon registration
Completion: Up to 52 weeks
Version Date: December 2011